Introduction: To study in-depth characteristics of single bicycle crashes and type of injuries considering gender and age differences. Methods: Hospital reported bicycle crashes identified in the Swedish national database STRADA were combined with self-reported detailed information regarding crash circumstances and injury outcomes. Gender and age-group differences were investigated using univariate statistics and Pearson Chi- Square test. Results: A total of 616 cyclists injured in single bicycle crashes between 2013 and 2017 were included. Participants (49% women) had a mean age of 58 years (ranged 15–89 years), most rode a comfort bike (54%) and cycled several times a week (81%). The most common crash type was skidding on ice or snow (26%). This crash type was significantly more common among women than men (30% versus 21%). Women more than twice as often lost balance at low or no speed (13% versus 5%). While men's injuries were located more than twice as often at shoulder and upper arm (28% versus 11%), women injured more than four times as often the lower leg and ankle (30% versus 7%). Differences regarding age-groups could be observed as an exponential increase of hip and upper leg injuries with increased age (9, 19 and 38%). Older cyclists were more often injured while losing balance at no or low speed and while getting on or off the bicycle. Conclusions: Concrete countermeasures to prevent injuries in single bicycle crashes can be suggested and directed to different target groups, i.e. women or men or younger or elderly.