In order to provide some countermeasures for alleviating severe traffic safety situation on expressways in China, a brief introduction to originality and development of road safety audit in developed countries is firstly provided in the paper. Then, the current practice of road safety audit on expressways in China is described. Also, the factors affecting the implementation of road safety audit are analyzed, and suggestions for promoting the application of road safety audit on expressways are proposed. Lastly, the development tendency of road safety audit on expressways is discussed and a case study based on both analysis of operating speed difference and expert experience is given.