The Multiple Stress Creep and Recovery test is recognised to better characterise the high-temperature property of bituminous binders than the traditional methods, especially for polymer modified bitumen (PmB). However, some experimental parameters may affect the test results, such as the preloading, measurement location, stress level. In this study, the effects of these parameters were evaluated through an interlaboratory experiment. Four bituminous binders were short-term aged and analysed by five different laboratories. The variability in most results met the requirements of the European standard, while some failed to pass the American precision criteria. The preloading at 0.1 kPa with ten cycles was found to have a recordable impact on PmB samples, but a less significant effect was observed for the unmodified ones. With the measurement locations, the influence is limited compared to other parameters. As for stress sensitivity, higher stresses lead to remarkable differences.