We apply the method presented by Johansson (2018) to estimate a volume delay function and marginal cost for bicycle traffic on cycling paths separated from motorized traffic based on point measurements of speed and lateral positions from seven sites in Sweden. The results indicate that a quadratic volume – delay function fits the data well in the observed range of volumes, and that there are significant delays already at volumes far below the capacity due to the heterogeneity of the desired speed over the population. The total marginal cost of delay per unit flow is estimated to €9×10-5 h/km.